The Agilent LC-QTOF 6530 is a high-resolution mass spectrometry system coupled with a high-performance liquid chromatography (HPLC) platform. This system is designed for the accurate identification of complex mixtures in various applications, including pharmaceuticals, environmental testing, and biomolecular analysis. It offers high sensitivity, fast data acquisition, and precise mass measurements, making it suitable for both qualitative and semi-quantitative analyses.
Key features
- HPLC System Model: Agilent 1290 Infinity II
• Flow Rate: 0.01–5.0 mL/min
• Maximum Pressure: up to 1200 bar
• Column Compartment Temperature Range: 4–110°C
• Autosampler Temperature Range: 4–40°C
• Solvent Compatibility: compatible with common organic solvents and aqueous - Mass Spectrometer Model: Agilent 6530 QTOF
• Ionization: Electrospray Ionization (ESI)
• Mass Range: 10–5000 m/z
• Mass Resolution: ~ 40,000 FWHM at m/z 922
• Mass Accuracy: <5 ppm with internal calibration
• Data Acquisition Modes: Full scan and MS/MS
• Scan Speed: up to 2 spectra/s Full scan, up to 5 spectra/s MS/MS - Typical Data Generated:
• High-resolution spectra suitable for identification of compounds in complex mixtures
The Agilent GC-QToF system is a high-resolution gas chromatography system coupled with quadrupole time-of-flight mass spectrometry, designed for the precise analysis of volatile and semi-volatile compounds. It offers superior mass accuracy, sensitivity, and resolution, making it ideal for advanced applications in environmental analysis, forensics, food safety, and chemical research. The system enables accurate identification and quantification of complex compound mixtures with high confidence.
Key features
- Gas Chromatograph (GC 8590)
• Temperature range: up to 450°C
• Fast oven ramping for quick analysis
• Equipped with high-performance HP-5ms column (non-polar, low bleed, 15 m × 0.250 mm internal diameter × 0.25 μm film thickness, max working temperature: 325 °C) - Mass Spectrometer (GC-QToF 7280) Ionization: EI (Electron Ionization)
• Mass Range: 10–1050 m/z
• Sensitivity: <2 ppm with internal reference
• Data Acquisition Mode: Full scan, MS/MS - Typical Data Generated
• Total Ion Chromatograms (TIC)
• Extracted Ion Chromatograms (EIC)
• High-resolution mass spectra with accurate mass measurements
• MS/MS fragmentation spectra for structure elucidation.
This analytical platform is designed for the detailed characterization of volatile and semi-volatile organic compounds in liquid samples and sample headspace. The technique separates complex mixtures in the gas phase and combines this separation with spectrometric identification and quantitative signal evaluation. The system supports both compound identification and concentration determination using chromatographic retention behavior, together with spectral library matching. A flame ionization detector complements the mass-selective detector and provides a robust quantitative response for organic species. Automated headspace sampling enables efficient analysis of volatile components released from complex matrices while minimizing sample preparation steps.
Key features
- GC–MS analysis of volatile and semi-volatile organic compounds
- Dual detection using mass spectrometry and a flame ionization detector
- Automated headspace sampling for volatile fractions
- Library-supported compound identification and calibrated quantification
- Suitable for VOCs, fuels, flavors, pesticides, and derivatized analytes
This configuration is focused on the measurement of gaseous samples and light volatile components, including permanent gases and low-molecular hydrocarbons. It enables the separation of gas mixtures followed by selective detection using multiple complementary detector types.
The platform combines mass-selective detection with thermal-conductivity and flame-ionization detectors to cover a broad range of gas-phase analytes. Permanent gases such as hydrogen and carbon monoxide are determined using thermal conductivity detection, while carbon dioxide and hydrocarbon species are evaluated by FID and mass spectrometry. The system supports both qualitative identification and calibration-based quantification in gas analysis workflows.
Key features
- Dedicated analysis of gaseous and highly volatile compounds
- Combined MS, TCD, and FID detection in one platform
- Suitable for H2, CO, CO2, hydrocarbons, and VOC gases
- Complementary identification and quantification strategies
- Reproducible, method-based gas analysis workflows
Liquid chromatography–mass spectrometry (LC–MS) enables detailed characterization of complex liquid samples that cannot be analyzed by gas-phase techniques. The method is particularly suitable for polar, non-volatile, and thermally unstable organic molecules, providing molecular- level insight alongside reliable quantitative data.
The LC–MS platform is used for routine and advanced analysis of organic compounds across a wide range of application areas. It supports the identification of unknown substances based on molecular ion signals and fragmentation patterns, as well as the accurate determination of concentrations using calibrated methods. The system is well-suited for purity evaluation, impurity and degradation profiling, and metabolite analysis in chemically complex matrices. Method-driven operation ensures high repeatability and analytical robustness.
Key features
- Qualitative and quantitative LC–MS analysis of polar and non-volatile compounds
- Suitable for thermally unstable and complex organic molecules
- Molecular mass–based compound identification (m/z)
- Purity testing and degradation/impurity profiling
- Applicable to pharmaceutical, biological, environmental, and food samples
